RNA

Part:BBa_K2654002:Design

Designed by: Alexandre Jolly   Group: iGEM18_UCLouvain   (2018-10-09)


sgRNA with a switchable crRNA sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 12
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 640
    Illegal AgeI site found at 752
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Verify that the four igem restriction sites were absent of the biobrick. We suppressed the DAM sequences to avoid potential methylations. Verification of the presence of a functional terminator. Verification that the sequence is a correct sgRNA according to the literature. In consequence, all the sequences were modified to correspond to the Biobrick standards and the iGEM requirements.


Source

A strong promoter J23119 produce the sgRNA and RFP

References